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Promega solubilization stop solution
Solubilization Stop Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+solution/stop+solubilization+solution/pmc12105100-133-4-7
Average 90 stars, based on 1 article reviews
solubilization stop solution - by Bioz Stars, 2026-09
90/100 stars

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MTT Assay:

Article Title: Oncogenic IL7R gain-of-function mutations in childhood T-cell acute lymphoblastic leukemia
Article Snippet: .. We added 8 ~l of MTT (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide; 5 mg/ml; Sigma) to each well and kept cells at 37 °C for 4 h, after which 100 ~l of solubilization solution (Promega) was added, and cells were incubated overnight at 37 °C. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide.
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 ◦ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 ◦ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 ◦ C. The absorbance was measured at 570 nm. ..

Article Title: The Anti-Aging and Tumor Suppressor Protein Klotho Enhances Differentiation of a Human Oligodendrocytic Hybrid Cell Line
Article Snippet: .. After 24 h, the medium was replaced with fresh DMEM with 10 % FBS or serum-free DMEM and cells were treated with recombinant Klotho at 0.4 μg/mL for 48 h. Following treatment, 15 μL of 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye (Promega) were added to cells, incubated for 3 h at 37 °C, then quenched with 100 μL of solubilization solution from the Promega kit for 30 min at room temperature. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. The absorbance was measured at 570 nm. ..

Incubation:

Article Title: Oncogenic IL7R gain-of-function mutations in childhood T-cell acute lymphoblastic leukemia
Article Snippet: .. We added 8 ~l of MTT (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide; 5 mg/ml; Sigma) to each well and kept cells at 37 °C for 4 h, after which 100 ~l of solubilization solution (Promega) was added, and cells were incubated overnight at 37 °C. ..

Article Title: Inhibiting Janus Kinase 1 and BCL-2 to treat T cell acute lymphoblastic leukemia with IL7-Rα mutations
Article Snippet: 15 μL of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; 5 mg/ml; Sigma) was added to each well and incubated at 37°C for 4 hours. .. 100 μl solubilization solution (Promega) was added to each well, and cells were incubated over night at 37°C. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide.
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 ◦ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 ◦ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 ◦ C. The absorbance was measured at 570 nm. ..

Article Title: Macrophages and Natural Killers Degrade α-Synuclein Aggregates.
Article Snippet: .. Next, solubilization solution (Promega, Madison, Wisconsin) was added and incubated for 1 h at 37 °C under 5% CO2. .. Absorption measurements were made in a plate reader (Tecan, Man̈nedorf, Switzerland) at 570 nm.

Article Title: Macrophages and Natural Killers Degrade α-Synuclein Aggregates
Article Snippet: .. Next, solubilization solution (Promega, Madison, Wisconsin) was added and incubated for 1 h at 37 °C under 5% CO 2 . .. Absorption measurements were made in a plate reader (Tecan, Männedorf, Switzerland) at 570 nm.

Article Title: The Anti-Aging and Tumor Suppressor Protein Klotho Enhances Differentiation of a Human Oligodendrocytic Hybrid Cell Line
Article Snippet: .. After 24 h, the medium was replaced with fresh DMEM with 10 % FBS or serum-free DMEM and cells were treated with recombinant Klotho at 0.4 μg/mL for 48 h. Following treatment, 15 μL of 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye (Promega) were added to cells, incubated for 3 h at 37 °C, then quenched with 100 μL of solubilization solution from the Promega kit for 30 min at room temperature. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. The absorbance was measured at 570 nm. ..

Transferring:

Article Title: Physiological Impact of a Synthetic Elastic Protein in Arterial Diseases Related to Alterations of Elastic Fibers: Effect on the Aorta of Elastin-Haploinsufficient Male and Female Mice
Article Snippet: After 1, 3 or 6 days, the cells were incubated for 4 h at 37 °C, 5% CO 2 with 15 μL Dye solution (cat. #G4101, Promega, Madison, WI, USA). .. Then, the insoluble formazan crystals were dissolved using 100 μL solubilization solution (cat. #G4102, Promega, Madison, WI, USA) for 1 h at room temperature; then, they were homogenized using a pipette, and the resulting colored solution was quantified by measuring the absorbance at 560 nm with a multi-well spectrophotometer (1681000, Bio-Rad, Marnes-la-Coquette, France). ..

Spectrophotometry:

Article Title: Physiological Impact of a Synthetic Elastic Protein in Arterial Diseases Related to Alterations of Elastic Fibers: Effect on the Aorta of Elastin-Haploinsufficient Male and Female Mice
Article Snippet: After 1, 3 or 6 days, the cells were incubated for 4 h at 37 °C, 5% CO 2 with 15 μL Dye solution (cat. #G4101, Promega, Madison, WI, USA). .. Then, the insoluble formazan crystals were dissolved using 100 μL solubilization solution (cat. #G4102, Promega, Madison, WI, USA) for 1 h at room temperature; then, they were homogenized using a pipette, and the resulting colored solution was quantified by measuring the absorbance at 560 nm with a multi-well spectrophotometer (1681000, Bio-Rad, Marnes-la-Coquette, France). ..

Variant Assay:

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide.
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 ◦ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 ◦ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 ◦ C. The absorbance was measured at 570 nm. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. The absorbance was measured at 570 nm. ..

Recombinant:

Article Title: The Anti-Aging and Tumor Suppressor Protein Klotho Enhances Differentiation of a Human Oligodendrocytic Hybrid Cell Line
Article Snippet: .. After 24 h, the medium was replaced with fresh DMEM with 10 % FBS or serum-free DMEM and cells were treated with recombinant Klotho at 0.4 μg/mL for 48 h. Following treatment, 15 μL of 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye (Promega) were added to cells, incubated for 3 h at 37 °C, then quenched with 100 μL of solubilization solution from the Promega kit for 30 min at room temperature. ..



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